• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

UNC13A Monoclonal antibody

UNC13A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C3A9

Cat no : 68483-1-Ig

Print datasheet

Synonyms

KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A

验证数据展示

  • WB analysis using 68483-1-Ig

    WB analysis using 68483-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68483-1-Ig (UNC13A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68483-1-Ig

    IHC staining of mouse brain using 68483-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68483-1-Ig (UNC13A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68483-1-Ig

    IHC staining of mouse brain using 68483-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68483-1-Ig (UNC13A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 68483-1-Ig

    IF Staining of SH-SY5Y using 68483-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using UNC13A antibody (68483-1-Ig, Clone: 3C3A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68483-1-Ig targets UNC13A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name unc-13 homolog A (C. elegans)
Synonyms KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A
Calculated Molecular Weight 193 kDa
Observed Molecular Weight193-210 kDa
GenBank Accession NumberNM_001080421
Gene Symbol UNC13A
Gene ID (NCBI) 23025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UPW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

实验方案

Product Specific Protocols
WB protocol for UNC13A antibody 68483-1-IgDownload protocol
IHC protocol for UNC13A antibody 68483-1-IgDownload protocol
IF protocol for UNC13A antibody 68483-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols