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验证数据展示

  • FC experiment of SH-SY5Y using CL488-68483

    FC experiment of SH-SY5Y using CL488-68483

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated UNC13A Monoclonal antibody (CL488-68483, Clone:3C3A9)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a isotype control (11A1B2) (CL488-66360-2, Clone: 11A1B2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of SH-SY5Y using CL488-68483

    IF Staining of SH-SY5Y using CL488-68483

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 UNC13A antibody (CL488-68483, Clone: 3C3A9 ) at dilution of 1:400.

CoraLite® Plus 488-conjugated UNC13A Monoclonal antibody

UNC13A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68483

产品说明书

CloneNo. 3C3A9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

IF/ICC, FC (Intra)

别名

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 68483-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68483 targets UNC13A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 unc-13 homolog A (C. elegans)
别名 Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032
计算分子量 193 kDa
观测分子量193-210 kDa
GenBank蛋白编号NM_001080421
基因名称 UNC13A
Gene ID (NCBI) 23025
RRIDAB_3673021
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UPW8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UNC13A antibody CL488-68483Download protocol
FC protocol for CL Plus 488 UNC13A antibody CL488-68483Download protocol
Standard Protocols
Click here to view our Standard Protocols