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验证数据展示

  • IF Staining of SH-SY5Y using CL555-68483

    IF Staining of SH-SY5Y using CL555-68483

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 UNC13A antibody (CL555-68483, Clone: 3C3A9 ) at dilution of 1:400.

  • FC experiment of SH-SY5Y using CL555-68483

    FC experiment of SH-SY5Y using CL555-68483

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated UNC13A Monoclonal antibody (CL555-68483, Clone:3C3A9)(red), or 0.4 ug CoraLite® Plus 555 Mouse IgG2a isotype control (11A1B2) (CL555-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®555-conjugated UNC13A Monoclonal antibody

UNC13A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-68483

产品说明书

CloneNo. 3C3A9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

IF/ICC, FC (Intra)

别名

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

68483-1-Ig

For other applications, we recommend the unconjugated version of this antibody, 68483-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-68483 targets UNC13A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 unc-13 homolog A (C. elegans)
别名 Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032
计算分子量 193 kDa
观测分子量193-210 kDa
GenBank蛋白编号NM_001080421
基因名称 UNC13A
Gene ID (NCBI) 23025
RRIDAB_3673423
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UPW8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

实验方案

Product Specific Protocols
IF protocol for CL555 UNC13A antibody CL555-68483Download protocol
FC protocol for CL555 UNC13A antibody CL555-68483Download protocol
Standard Protocols
Click here to view our Standard Protocols