验证数据展示
经过测试的应用
Positive WB detected in | U2OS cells, MCF-7 cells, K-562 cells, HepG2 cells, HeLa cells, HEK-293 cells |
Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84877-5-RR targets WAPL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | WAPL fusion protein Ag9585 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | wings apart-like homolog (Drosophila) |
别名 | WAPL/FOE, FOE, 242443C1, WAPL cohesin release factor, WAPAL |
计算分子量 | 142 kDa |
观测分子量 | 160-180 kDa |
GenBank蛋白编号 | BC017393 |
基因名称 | WAPL |
Gene ID (NCBI) | 23063 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q7Z5K2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for WAPL antibody 84877-5-RR | Download protocol |
IHC protocol for WAPL antibody 84877-5-RR | Download protocol |
IF protocol for WAPL antibody 84877-5-RR | Download protocol |
FC protocol for WAPL antibody 84877-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |