• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82870-7-RR (same clone as 82870-7-PBS)

    WB analysis using 82870-7-RR (same clone as 82870-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 82870-7-RR (same clone as 82870-7-PBS)

    IHC staining of mouse kidney using 82870-7-RR (same clone as 82870-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82870-7-RR (same clone as 82870-7-PBS)

    IF Staining of U2OS using 82870-7-RR (same clone as 82870-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

    IF Staining of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

    FC experiment of MCF-7 using 82870-7-RR (same clone as 82870-7-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZO-1 Recombinant antibody (82870-7-RR, Clone:240150D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 82870-7-RR (same clone as 82870-7-PBS)

    IP experiment of HEK-293 using 82870-7-RR (same clone as 82870-7-PBS)

    IP result of anti-ZO-1 (IP:82870-7-RR, 4ug; Detection:82870-7-RR 1:4000) with HEK-293 cells lysate 1610 ug. This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82870-7-RR (same clone as 82870-7-PBS)

    IF Staining of U2OS using 82870-7-RR (same clone as 82870-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82870-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82870-7-RR

    Affinity and Kinetic Characterization of 82870-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82870-7-RR against Human ZO-1 were performed. The affinity constant is 16.2 nM.

ZO-1 Recombinant antibody, PBS Only

ZO-1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 82870-7-PBS

产品说明书

CloneNo. 240150D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

82870-7-PBS targets ZO-1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原 ZO-1 fusion protein Ag33182 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tight junction protein 1 (zona occludens 1)
别名 TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1
计算分子量 1748 aa, 195 kDa
观测分子量 230 kDa
GenBank蛋白编号BC111712
基因名称 ZO-1
Gene ID (NCBI) 7082
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ07157
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).