ZO-1 Recombinant antibody
ZO-1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
240150D7
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells, Caco-2 cells, HT-29 cells, MCF-7 cells, rat brain tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells, U2OS cells, MDCK cells, Caco-2 cells |
Positive FC (Intra) detected in | MCF-7 cells |
Freshly prepared samples are recommended.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 82870-7-RR is ZO-1 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat, canine |
Immunogen | ZO-1 fusion protein Ag33182 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | tight junction protein 1 (zona occludens 1) |
Synonyms | TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1 |
Calculated Molecular Weight | 1748 aa, 195 kDa |
Observed Molecular Weight | 230 kDa |
GenBank Accession Number | BC111712 |
Gene Symbol | ZO-1 |
Gene ID (NCBI) | 7082 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q07157 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ZO-1 antibody 82870-7-RR | Download protocol |
IHC protocol for ZO-1 antibody 82870-7-RR | Download protocol |
IF protocol for ZO-1 antibody 82870-7-RR | Download protocol |
IP protocol for ZO-1 antibody 82870-7-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |