CoraLite®594-conjugated ZO-1 Recombinant antibody
ZO-1 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
240150D7
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Freshly prepared samples are recommended.
For other applications, we recommend the unconjugated version of this antibody, 82870-7-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-82870-7 targets ZO-1 in IF/ICC applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat, canine |
| Immunogen | ZO-1 fusion protein Ag33182 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | tight junction protein 1 (zona occludens 1) |
| Synonyms | |
| Calculated Molecular Weight | 1748 aa, 195 kDa |
| Observed Molecular Weight | 230 kDa |
| GenBank Accession Number | BC111712 |
| Gene Symbol | ZO-1 |
| Gene ID (NCBI) | 7082 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q07157 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 ZO-1 antibody CL594-82870-7 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |