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磷酸化抗体

Phosphorylated antibodies

 

蛋白质磷酸化修饰是指通过蛋白质磷酸激酶将ATP分子中的磷酸基转移到蛋白质分子特定位点（Ser、Thr、Tyr残基等）的过程。这种蛋白修饰在调控信号转导、基因表达、细胞周期、发育分化等诸多过程中起着至关重要的作用。

而磷酸化抗体主要是针对磷酸化位点制备的，可以特异性识别磷酸化氨基酸位点，对磷酸化蛋白进行定性、定量分析，检测蛋白受到刺激时磷酸化水平变化情况，侧重于了解该蛋白的一种活性状态。因此，在磷酸化抗体研发的过程中，选择合适的刺激条件，是磷酸化蛋白检测中至关重要的一步。

Proteintech自主研发生产的磷酸化抗体，经过多种样本及多重实验验证，有效确保产品稳定性及检测数据可重复性。

 

点击查看Proteintech磷酸化抗体

 

▲Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

 

 

 

▲Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using Phospho-STK11 (Thr189) antibody (80127-1-RR, Clone: 5J9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

▲1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®488 Anti-Human Phospho-STK11 (Thr189) (CL488-80127, Clone:5J9), or 0.13 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.