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- KD/KO Validated
Cyclin A2 Polyclonal antibody
Cyclin A2 Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, FC (Intra), IP, ELISA and More (1)
Conjugate
Unconjugated
178
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, K-562 cells, U-937 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells |
Positive IP detected in | HeLa cells, HL-60 cells |
Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, MCF-7 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 170 publications below |
CoIP | See 1 publications below |
产品信息
18202-1-AP targets Cyclin A2 in WB, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, CoIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig, monkey |
Immunogen | Cyclin A2 fusion protein Ag12765 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cyclin A2 |
Synonyms | CCNA2, CyclinA2, Cyclin-A, Cyclin A, CCNA |
Calculated Molecular Weight | 432 aa, 49 kDa |
Observed Molecular Weight | 48-52 kDa |
GenBank Accession Number | BC104783 |
Gene Symbol | Cyclin A2 |
Gene ID (NCBI) | 890 |
RRID | AB_10597084 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20248 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Cyclin A2 antibody 18202-1-AP | Download protocol |
IHC protocol for Cyclin A2 antibody 18202-1-AP | Download protocol |
IF protocol for Cyclin A2 antibody 18202-1-AP | Download protocol |
IP protocol for Cyclin A2 antibody 18202-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Metab LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration | ||
Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis | ||
Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. | ||
Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. | ||
Sci Adv The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis |