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  • Featured Product
  • KD/KO Validated

P27; KIP1 Polyclonal antibody

P27; KIP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 26714-1-AP

Print datasheet

Synonyms

CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27

验证数据展示

  • WB analysis of NIH/3T3 using 26714-1-AP

    WB analysis of NIH/3T3 using 26714-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 26714-1-AP

    WB analysis of HeLa using 26714-1-AP

    WB result of P27; KIP1 antibody (26714-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P27; KIP1 transfected HeLa cells.

  • IHC staining of human gliomas using 26714-1-AP

    IHC staining of human gliomas using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDKN1B antibody included
  • IHC staining of human gliomas using 26714-1-AP

    IHC staining of human gliomas using 26714-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDKN1B antibody included
  • IF Staining of MCF-7 using 26714-1-AP

    IF Staining of MCF-7 using 26714-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 26714-1-AP

    FC experiment of MCF-7 using 26714-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human P27; KIP1 (26714-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using 26714-1-AP

    IF Staining of HepG2 using 26714-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P27; KIP1 antibody (26714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inNIH/3T3 cells, MCF-7 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue, human tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDKN1B Ready-To-Use IHC Kit. CDKN1B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

26714-1-AP targets P27; KIP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen P27; KIP1 fusion protein Ag25083 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Synonyms CDKN1B, P27, MEN1B, KIP1, Cyclin-dependent kinase inhibitor p27
Calculated Molecular Weight 198 aa, 22 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC001971
Gene Symbol p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
RRIDAB_2880611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46527
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

实验方案

Product Specific Protocols
WB protocol for P27; KIP1 antibody 26714-1-APDownload protocol
IHC protocol for P27; KIP1 antibody 26714-1-APDownload protocol
IF protocol for P27; KIP1 antibody 26714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia
View Article
humanWB

Biosci Rep

C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo.

Authors - Wei Zhang
View Article