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- KD/KO Validated
Cyclin B1 Polyclonal antibody
Cyclin B1 Polyclonal Antibody for WB, IP, IF/ICC, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (4)
Applications
WB, IP, IF/ICC, FC, ELISA and More (1)
Conjugate
Unconjugated
244
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IF detected in | HeLa cells, U2OS cells |
| Positive FC detected in | HeLa cells, Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy CCNB1 Ready-To-Use IHC Kit. CCNB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55004-1-AP targets Cyclin B1 in WB, IP, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IF/ICC, FC, ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, sheep, pig, bovine |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin B1 |
| Synonyms | CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | NM_031966 |
| Gene Symbol | CCNB1 |
| Gene ID (NCBI) | 891 |
| RRID | AB_10859790 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14635 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin B1 antibody 55004-1-AP | Download protocol |
| IF protocol for Cyclin B1 antibody 55004-1-AP | Download protocol |
| IP protocol for Cyclin B1 antibody 55004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration | ||
Acta Pharm Sin B Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway. | ||
Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. | ||
Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. | ||
Autophagy PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma. | ||
Cell Death Dis HDAC7/c-Myc signaling pathway promotes the proliferation and metastasis of choroidal melanoma cells |
