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VDAC1/2 Polyclonal antibody
VDAC1/2 Polyclonal Antibody for IF, IHC, WB, ELISA
Cat No : 10866-1-AP
|Positive WB detected in||HEK-293 cells, 4T1 cells, DU 145 cells, HeLa cells, HL-60 cells, human heart tissue, mouse brain tissue, mouse kidney tissue, MCF-7 cells, MDA-MB-453s cells, mouse heart tissue, rat heart tissue, Jurkat cells, rat kidney tissue|
|Positive IHC detected in||human colon tissue, human pancreas tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10866-1-AP targets VDAC1/2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||IF, IHC, WB, ELISA|
|Cited Applications||CoIP, IF, IHC, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Pieris rapae, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VDAC1/2 fusion protein Ag1144|
|Full Name||voltage-dependent anion channel 1|
|Synonyms||hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1, VDAC2|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC008482|
|Uniprot||P21796||Gene ID (NCBI)||7416|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2.
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