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  • Featured Product
  • KD/KO Validated

VEGFA Polyclonal antibody

VEGFA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 19003-1-AP

Print datasheet

Synonyms

VEGF, Vascular permeability factor, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor

验证数据展示

  • WB analysis using 19003-1-AP

    WB analysis using 19003-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 19003-1-AP

    IHC staining of human lung cancer using 19003-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VEGFA/VEGF antibody included
  • IF Staining of HeLa using 19003-1-AP

    IF Staining of HeLa using 19003-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human lung cancer using 19003-1-AP

    IHC staining of human lung cancer using 19003-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VEGFA/VEGF antibody included
  • FC experiment of NIH/3T3 using 19003-1-AP

    FC experiment of NIH/3T3 using 19003-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human VEGFA (19003-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 19003-1-AP

    WB analysis using 19003-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 19003-1-AP

    IP experiment of Jurkat using 19003-1-AP

    IP result of anti-VEGFA (IP:19003-1-AP, 4ug; Detection:19003-1-AP 1:3000) with Jurkat cells lysate 1560 ug.

  • IF Staining of HeLa using 19003-1-AP

    IF Staining of HeLa using 19003-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, C6 cells, Jurkat cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19003-1-AP targets VEGFA in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, rabbit, monkey, sheep, goat
Immunogen VEGFA fusion protein Ag13500 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms VEGF, Vascular permeability factor, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor
Calculated Molecular Weight 412 aa, 46 kDa
Observed Molecular Weight36-40 kDa
GenBank Accession NumberBC065522
Gene Symbol VEGFA
Gene ID (NCBI) 7422
RRIDAB_2212657
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15692
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

实验方案

Product Specific Protocols
WB protocol for VEGFA antibody 19003-1-APDownload protocol
IHC protocol for VEGFA antibody 19003-1-APDownload protocol
IF protocol for VEGFA antibody 19003-1-APDownload protocol
IP protocol for VEGFA antibody 19003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nat Nanotechnol

Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.

Authors - Xuefei Lu
  • KD Validated
View Article
human

Bioact Mater

A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds

Authors - Shunlai Shang
View Article
mouse,human

ACS Nano

Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.

Authors - Meng Zhao
View Article
mouseIHC

Biomaterials

A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration

Authors - Congcong Yu
View Article
mouse

Light Sci Appl

Microglia modulation with 1070-nm light attenuates Aβ burden and cognitive impairment in Alzheimer's disease mouse model.

Authors - Lechan Tao
View Article
humanIP

J Clin Invest

Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.

Authors - Akrit Sodhi
View Article