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  • Featured Product
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ANGPTL4 Polyclonal antibody

ANGPTL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18374-1-AP

Print datasheet

Synonyms

ANGPTL2, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Angiopoietin related protein 4, Angiopoietin like protein 4

验证数据展示

  • WB analysis using 18374-1-AP

    WB analysis using 18374-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 18374-1-AP

    WB analysis of mouse kidney using 18374-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18374-1-AP (ANGPTL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18374-1-AP

    IHC staining of human kidney using 18374-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 18374-1-AP

    IHC staining of human oesophagus cancer using 18374-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 18374-1-AP

    IHC staining of human oesophagus cancer using 18374-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18374-1-AP (ANGPTL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse heart using 18374-1-AP

    IP experiment of mouse heart using 18374-1-AP

    IP result of anti-ANGPTL4 (IP:18374-1-AP, 4ug; Detection:18374-1-AP 1:500) with mouse heart tissue lysate 4000ug.

  • IF Staining of NIH/3T3 using 18374-1-AP

    IF Staining of NIH/3T3 using 18374-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HT-29 using 18374-1-AP

    IF Staining of HT-29 using 18374-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HEK-293 using 18374-1-AP

    IF Staining of HEK-293 using 18374-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ANGPTL4 antibody (18374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, HEK-293 cells, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman kidney tissue, human oesophagus cancer tissue, human heart tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, HT-29 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18374-1-AP targets ANGPTL4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ANGPTL4 fusion protein Ag13237 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name angiopoietin-like 4
Synonyms ANGPTL2, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Angiopoietin related protein 4, Angiopoietin like protein 4
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC023647
Gene Symbol ANGPTL4
Gene ID (NCBI) 51129
RRIDAB_2878539
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

实验方案

Product Specific Protocols
WB protocol for ANGPTL4 antibody 18374-1-APDownload protocol
IHC protocol for ANGPTL4 antibody 18374-1-APDownload protocol
IF protocol for ANGPTL4 antibody 18374-1-APDownload protocol
IP protocol for ANGPTL4 antibody 18374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Metab

Resistant starch intake facilitates weight loss in humans by reshaping the gut microbiota

Authors - Huating Li
View Article
humanIP

J Clin Invest

Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.

Authors - Akrit Sodhi
View Article
mouseWB

EMBO J

LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Authors - Yanying Wang
View Article
mouseIF

J Pathol

ANGPTL4-αvβ3 integrin interaction counteracts hypoxia-induced vascular permeability by modulating Src signalling downstream of VEGFR2.

Authors - Elisa Gomez Perdiguero
View Article
humanIHC

Breast Cancer Res

An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-beta in human breast cancer.

Authors - Misako Sato
View Article
humanIHC,WB

J Cell Mol Med

Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure.

Authors - Mingyang Li
View Article