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- KD/KO Validated
HIF-1 alpha Polyclonal antibody
HIF-1 alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (5)
Conjugate
Unconjugated
654
验证数据展示
经过测试的应用
| Positive WB detected in | Cobalt Chloride treated HeLa cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
Samples need to be treated with hypoxia stimulation.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20960-1-AP targets HIF-1 alpha in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, Cell treatment applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP, ChIP, RIP, Cell treatment |
| Tested Reactivity | human |
| Cited Reactivity | human, pig, chicken, goat |
| Immunogen | HIF-1 alpha fusion protein Ag15198 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Synonyms | HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1 |
| Calculated Molecular Weight | 826 aa, 93 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC012527 |
| Gene Symbol | HIF1A |
| Gene ID (NCBI) | 3091 |
| RRID | AB_10732601 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16665 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HIF-1 alpha antibody 20960-1-AP | Download protocol |
| IHC protocol for HIF-1 alpha antibody 20960-1-AP | Download protocol |
| IF protocol for HIF-1 alpha antibody 20960-1-AP | Download protocol |
| IP protocol for HIF-1 alpha antibody 20960-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
Cancer Cell Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity | ||
Nat Cell Biol A single-cell transcriptomic landscape of the lungs of patients with COVID-19. | ||
ACS Nano Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling | ||
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis |
