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验证数据展示

  • IF Staining of HeLa using CL647-20960

    IF Staining of HeLa using CL647-20960

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using CoraLite® Plus 647 HIF-1 alpha antibody (CL647-20960) at dilution of 1:200.

  • FC experiment of HeLa using CL647-20960

    FC experiment of HeLa using CL647-20960

    1x10^6 HeLa cells untreated (dashed lines) or treated with Cobalt Chloride (red) were intracellularly stained with 0.25 ug CoraLite® Plus 647-conjugated HIF-1 alpha Polyclonal antibody (CL647-20960), or 0.25 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated HIF-1 alpha Polyclonal antibody

HIF-1 alpha Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-20960

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCobalt Chloride treated HeLa cells
Positive FC (Intra) detected inCobalt Chloride treated HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 20960-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-20960 targets HIF-1 alpha in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 HIF-1 alpha fusion protein Ag15198 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
别名 HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1
计算分子量 826 aa, 93 kDa
观测分子量 120 kDa
GenBank蛋白编号BC012527
基因名称 HIF1A
Gene ID (NCBI) 3091
RRIDAB_2934944
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16665
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 HIF-1 alpha antibody CL647-20960Download protocol
Standard Protocols
Click here to view our Standard Protocols