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验证数据展示

  • WB analysis of human plasma using 66509-1-Ig

    WB analysis of human plasma using 66509-1-Ig

    human plasma was subjected to SDS PAGE followed by western blot with 66509-1-Ig (Coagulation Factor II antibody) at various dilutions incubated at room temperature for 1.5 hours.

  • WB analysis of human plasma using 66509-1-Ig

    WB analysis of human plasma using 66509-1-Ig

    Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • IHC staining of human liver using 66509-1-Ig

    IHC staining of human liver using 66509-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:150 (under 10x lens).

  • IHC staining of human liver using 66509-1-Ig

    IHC staining of human liver using 66509-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:150 (under 40x lens).

Coagulation Factor II Monoclonal antibody

Coagulation Factor II Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66509-1-Ig

产品说明书

CloneNo. 1E12A8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

Prothrombin, F2, EC:3.4.21.5, Activation peptide fragment 2, Activation peptide fragment 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66509-1-Ig targets Coagulation Factor II in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Coagulation Factor II fusion protein Ag18385 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coagulation factor II (prothrombin)
别名 Prothrombin, F2, EC:3.4.21.5, Activation peptide fragment 2, Activation peptide fragment 1
计算分子量 622 aa, 70 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC051332
基因名称 F2
Gene ID (NCBI) 2147
RRIDAB_2881872
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00734
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II, also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.

实验方案

Product Specific Protocols
WB protocol for Coagulation Factor II antibody 66509-1-IgDownload protocol
IHC protocol for Coagulation Factor II antibody 66509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols