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Alpha 2-Antiplasmin Monoclonal antibody

Alpha 2-Antiplasmin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3F6C3

Cat no : 67308-1-Ig

Print datasheet

Synonyms

A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, API, PLI, Serpin F2, SERPINF2

验证数据展示

  • WB analysis of HepG2 using 67308-1-Ig

    WB analysis of HepG2 using 67308-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67308-1-Ig

    IHC staining of human liver using 67308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 67308-1-Ig

    IHC staining of human liver using 67308-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67308-1-Ig

    IF Staining of HepG2 using 67308-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human plasma, THP-1 cells, K-562 cells, HuH-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67308-1-Ig targets Alpha 2-Antiplasmin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Alpha 2-Antiplasmin fusion protein Ag4095 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Synonyms A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, API, PLI, Serpin F2, SERPINF2
Calculated Molecular Weight 491 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC031592
Gene Symbol Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_2882569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08697
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
IHC protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
IF protocol for Alpha 2-Antiplasmin antibody 67308-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols