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NUP85 Recombinant antibody

NUP85 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240116A2

Cat no : 83288-1-RR

Print datasheet

Synonyms

Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT, 85 kDa nucleoporin

验证数据展示

  • IF Staining of HepG2 using 83288-1-RR

    IF Staining of HepG2 using 83288-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUP85 antibody (83288-1-RR, Clone: 240116A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of MCF-7 using 83288-1-RR

    FC experiment of MCF-7 using 83288-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NUP85 Recombinant antibody (83288-1-RR, Clone:240116A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy NUP85 Ready-To-Use IHC Kit. NUP85 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83288-1-RR is NUP85 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NUP85 fusion protein Ag7086 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nucleoporin 85kDa
Synonyms Nucleoporin Nup85, Nucleoporin Nup75, nucleoporin 85kDa, FROUNT, 85 kDa nucleoporin
Calculated Molecular Weight 75 kDa
GenBank Accession NumberBC000697
Gene Symbol NUP85
Gene ID (NCBI) 79902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BW27
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. NPCs are key for nucleocytoplasmic transport, but also regulate the mitotic machinery, transcription and chromatin organization through transport-independent mechanisms. The NUP107-160 complex is acknowledged to contribute to the assembly and maintenance of the NPC structure. Members of this largest subcomplex of the NPC associate with kinetochores, mitotic spindles, centrosomes and mitotic checkpoint regulators for proper completion of the cell cycle . Downregulation of NUP107-160 subcomplex members resulted in defective cytokinesis, compromised microtubule structures, altered cytoskeletal dynamics, impaired chromosome segregation and differentiation. (PMID:34170319)

实验方案

Product Specific Protocols
IF protocol for NUP85 antibody 83288-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols