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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-9 protein

Activity

0.1-0.6 ng/mL

Specific Activity

minimally 2.00 x 106 IU/mg

Species Reactivity

human

Purity

>95%

Cat No : HZ-1240

Print datasheet

Synonyms

Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40

Validation Data Gallery

  • Purity of recombinant human IL-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human IL-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human IL-9 (HZ-1240) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of recombinant IL-9 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.15-0.85 ng/mL​.

    Recombinant human IL-9 (HZ-1240) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of recombinant IL-9 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.15-0.85 ng/mL​.

Technical Specifications

GeneID 3578
Species Human
Expression HEK293
Activity 0.1-0.6 ng/mL
Specific Activityminimally 2.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP15248
Molecular Mass 38 to 45 kDa reduced, 32 to 40 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interleukin 9 (IL-9) is a γc-family cytokine produced by T-helper 9 (Th9) cells. IL-9 promotes the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the its cognate receptor (IL9R), which activates different signal transducer and activator proteins to execute various biological processes (PMID: 29703631; 29437190; 29392411; 29391143)

Synonyms

Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40

Publications

SpeciesTitle
human

Nature

MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment.

Authors - Vincenzo Calvanese
View Article
human

J Toxicol Sci

Human induced pluripotent stem cell-derived mast cells useful for in vitro mast cell activation assay exhibiting phenotypes and morphological characteristics of human mast cells.

Authors - Tatsuya Ikuno
View Article