ChromoTek 3xDYKDDDDK-peptide

HPLC-purified 3xDYKDDDDK-peptide

Peptide

DYKDDDDK tag

Applications

Elution

Conjugate

Unconjugated

Cat No : fp

Synonyms

DYKDDDDK peptide, DYKDDDDK, Flag



Product Information

HPLC-purified 3xDYKDDDDK-peptide

Description3xDYKDDDDK-peptide, also known as 3xFlag®-peptide
ApplicationsMild elution of DYKDDDDK-tagged fusion proteins bound to DYKDDDDK Fab-Trap or anti-DYKDDDDK antibodies.
SequenceMDYKDHDG DYKDHDI DYKDDDDK
FormLyophilized powder
ConjugateUnconjugated
Molecular weight2,862 g/mol
Purity>96% (HPLC)
ReconstitutionDissolve 1 mg 3xDYKDDDDK-peptide in 0.5 mL PBS pH 7.4 to a final concentration of 2 mg/mL (0.7 mM). Vortex for a few minutes to dissolve the powder. Aliquot upon reconstitution.
DilutionDilute 3xDYKDDDDK-peptide stock to 150 µg/mL (0.05 mM) in PBS for elution of DYKDDDDK-tagged fusion proteins.
Storage ConditionUpon receipt store at -20°C/-4°F.
Aliquot upon reconstitution and store at -20°C/-4°F.
Avoid repeated freeze / thaw cycles.
StabilityPowder stable for 1 year upon receipt
ShipmentShipped at ambient temperature

Publications

ApplicationTitle
CoIP

Curr Biol

Evolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria

Authors - Jovelyn Unay