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ChromoTek 3xDYKDDDDK-peptide

HPLC-purified 3xDYKDDDDK-peptide

Peptide

DYKDDDDK tag

Applications

Elution

Conjugate

Unconjugated

Cat No : fp

Synonyms

DYKDDDDK peptide, DYKDDDDK, Flag

Validation Data Gallery

  • DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

    DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.

Product Information

HPLC-purified 3xDYKDDDDK-peptide

Description3xDYKDDDDK-peptide, also known as 3xFlag®-peptide
ApplicationsMild elution of DYKDDDDK-tagged fusion proteins bound to DYKDDDDK Fab-Trap or anti-DYKDDDDK antibodies.
SequenceMDYKDHDG DYKDHDI DYKDDDDK
FormLyophilized powder
ConjugateUnconjugated
Molecular weight2,862 g/mol
Purity>96% (HPLC)
ReconstitutionDissolve 1 mg 3xDYKDDDDK-peptide in 0.5 mL PBS pH 7.4 to a final concentration of 2 mg/mL (0.7 mM). Vortex for a few minutes to dissolve the powder. Aliquot upon reconstitution.
DilutionDilute 3xDYKDDDDK-peptide stock to 150 µg/mL (0.05 mM) in PBS for elution of DYKDDDDK-tagged fusion proteins.
Storage ConditionUpon receipt store at -20°C/-4°F.
Aliquot upon reconstitution and store at -20°C/-4°F.
Avoid repeated freeze / thaw cycles.
StabilityPowder stable for 1 year upon receipt
ShipmentShipped at ambient temperature

Publications

ApplicationTitle
CoIP

Curr Biol

Evolution of paralogous multicomponent systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria

Authors - Jovelyn Unay
View Article