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5-Formylcytosine (5-fC) antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Not Species Specific

Applications

DB, ICC, IF, WB

Cat No : 61227,61228 61227

Synonyms

5-Formylcytidine, 5-formylcytosine, 5-fC, 5fc, TET, dna methylation, formylcytosine, stem cell, stem cells, sample

验证数据展示

  • 5-Formylcytosine antibody (pAb) tested by DNA dot blot Single-stranded 38 nt DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) corresponding to the immunogen and related sequences were spotted onto nitrocellulose and probed with the antibody at 2 ug/ml. Lane 1: oligo containing unmodified cytidine. Lane 2: oligo containing 5-methylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane 4: oligo containing 5-formylcytidine. Lane 5: oligo containing 5-carboxylcytidine.

    5-Formylcytosine antibody (pAb) tested by DNA dot blot Single-stranded 38 nt DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) corresponding to the immunogen and related sequences were spotted onto nitrocellulose and probed with the antibody at 2 ug/ml. Lane 1: oligo containing unmodified cytidine. Lane 2: oligo containing 5-methylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane 4: oligo containing 5-formylcytidine. Lane 5: oligo containing 5-carboxylcytidine.

产品信息

Tested Applications DB, ICC, IF, WB

Applications Validated by Active Motif: DB: 0.5 - 2 ug/ml dilution
Tested Reactivity Not Species Specific
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
ModificationMethylated
Immunogen This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.
Full Name 5-Formylcytosine (5-fC) antibody (pAb)
Synonyms 5-Formylcytidine, 5-formylcytosine, 5-fC, 5fc, TET, dna methylation, formylcytosine, stem cell, stem cells, sample
Molecular weight
GenBank accession numberN/A
RRIDAB_2793558
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.