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  • Featured Product
  • KD/KO Validated

AIF Monoclonal antibody, PBS Only

AIF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, canine

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8C12B2

Cat no : 67791-1-PBS

Print datasheet

Synonyms

AIFM1, PDCD8, EC:1.6.99.-, Apoptosis-inducing factor 1, mitochondrial

验证数据展示

  • WB analysis using 67791-1-Ig (same clone as 67791-1-PBS)

    WB analysis using 67791-1-Ig (same clone as 67791-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67791-1-Ig (AIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM1 antibody included
  • IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM1 antibody included
  • IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    WB analysis of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    WB result of AIF antibody (67791-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIF transfected HeLa cells. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AIFM1 Ready-To-Use IHC Kit. AIFM1 primary antibody included.

产品信息

67791-1-PBS targets AIF in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, canine
Immunogen AIF fusion protein Ag12400 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Synonyms AIFM1, PDCD8, EC:1.6.99.-, Apoptosis-inducing factor 1, mitochondrial
Calculated Molecular Weight 609 aa, 66 kDa
Observed Molecular Weight 67 kDa, 57 kDa
GenBank Accession NumberBC111065
Gene Symbol AIF
Gene ID (NCBI) 9131
RRIDAB_2918555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95831
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).