验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80816 targets AKT1-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | AKT1-Specific fusion protein Ag0213 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Synonyms | AKT pan, AKT 1, AKT (pan), AKT, AKT1 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56-62 kDa |
| GenBank Accession Number | BC000479 |
| Gene Symbol | AKT1 |
| Gene ID (NCBI) | 207 |
| RRID | AB_3673039 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P31749 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 AKT1-Specific antibody CL488-80816 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |