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  • Featured Product
  • KD/KO Validated

AKT1-Specific Recombinant antibody, PBS Only

AKT1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5O9

Cat no : 80816-1-PBS

Print datasheet

Synonyms

AKT pan, AKT 1, AKT (pan), AKT, AKT1

验证数据展示

  • WB analysis using 80816-1-RR (same clone as 80816-1-PBS)

    WB analysis using 80816-1-RR (same clone as 80816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • WB analysis of MCF-7 using 80816-1-RR (same clone as 80816-1-PBS)

    WB analysis of MCF-7 using 80816-1-RR (same clone as 80816-1-PBS)

    WB result of AKT1 antibody (80816-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 80816-1-RR (same clone as 80816-1-PBS)

    IP experiment of HEK-293 using 80816-1-RR (same clone as 80816-1-PBS)

    IP result of anti-AKT1 (IP:80816-1-RR, 4ug; Detection:80816-1-RR 1:2000) with HEK-293 cells lysate 1280 ug. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 80816-1-RR (same clone as 80816-1-PBS)

    FC experiment of Jurkat using 80816-1-RR (same clone as 80816-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80816-1-RR

    Affinity and Kinetic Characterization of 80816-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80816-1-RR against Human AKT1 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

80816-1-PBS targets AKT1-Specific in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AKT1-Specific fusion protein Ag0213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms AKT pan, AKT 1, AKT (pan), AKT, AKT1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-62 kDa
GenBank Accession NumberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. 80816-1-RR recognizes AKT1 specifically.