• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80816-1-RR (same clone as 80816-1-PBS)

    WB analysis using 80816-1-RR (same clone as 80816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKT antibody included
  • WB analysis of MCF-7 using 80816-1-RR (same clone as 80816-1-PBS)

    WB analysis of MCF-7 using 80816-1-RR (same clone as 80816-1-PBS)

    WB result of AKT1 antibody (80816-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 80816-1-RR (same clone as 80816-1-PBS)

    IP experiment of HEK-293 using 80816-1-RR (same clone as 80816-1-PBS)

    IP result of anti-AKT1 (IP:80816-1-RR, 4ug; Detection:80816-1-RR 1:2000) with HEK-293 cells lysate 1280 ug. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 80816-1-RR (same clone as 80816-1-PBS)

    FC experiment of Jurkat using 80816-1-RR (same clone as 80816-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

AKT1-Specific Recombinant antibody, PBS Only

AKT1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 80816-1-PBS

产品说明书

CloneNo. 5O9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

AKT pan, AKT 1, AKT (pan), AKT, AKT1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

80816-1-PBS targets AKT1-Specific in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AKT1-Specific fusion protein Ag0213 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 1
别名 AKT pan, AKT 1, AKT (pan), AKT, AKT1
计算分子量 56 kDa
观测分子量 56-62 kDa
GenBank蛋白编号BC000479
基因名称 AKT1
Gene ID (NCBI) 207
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31749
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. 80816-1-RR recognizes AKT1 specifically.