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AKT1-Specific Recombinant antibody
AKT1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
5O9
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, MCF-7 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells, PC-12 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
80816-1-RR targets AKT1-Specific in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | AKT1-Specific fusion protein Ag0213 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Synonyms | AKT pan, AKT 1, AKT (pan), AKT, AKT1 |
Calculated Molecular Weight | 56 kDa |
Observed Molecular Weight | 56-62 kDa |
GenBank Accession Number | BC000479 |
Gene Symbol | AKT1 |
Gene ID (NCBI) | 207 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P31749 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for AKT1-Specific antibody 80816-1-RR | Download protocol |
IHC protocol for AKT1-Specific antibody 80816-1-RR | Download protocol |
IP protocol for AKT1-Specific antibody 80816-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int Immunopharmacol Geniposide ameliorates psoriatic skin inflammation by inhibiting the TLR4/MyD88/NF-κB p65 signaling pathway and MMP9 |