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验证数据展示

  • WB analysis using 29373-1-AP

    WB analysis using 29373-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29373-1-AP (ALDH1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 29373-1-AP

    IHC staining of human liver cancer using 29373-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29373-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ALDH1A3 Polyclonal antibody

ALDH1A3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29373-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

Aldehyde dehydrogenase 6, ALDH1A3, ALDH1A6, ALDH6, RALDH 3, RALDH3, Retinaldehyde dehydrogenase 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29373-1-AP targets ALDH1A3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen ALDH1A3 fusion protein Ag31100 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldehyde dehydrogenase 1 family, member A3
Synonyms Aldehyde dehydrogenase 6, ALDH1A3, ALDH1A6, ALDH6, RALDH 3, RALDH3, Retinaldehyde dehydrogenase 3
Calculated Molecular Weight 512 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC069274
Gene Symbol ALDH1A3
Gene ID (NCBI) 220
RRIDAB_2918287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47895
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDH1A3 is the predominant ALDH isozyme that participates in various physiological processes in human cells. Growing evidence has shown that ALDH1A3 exhibits high activity in cancer stem cells (CSCs) and influences a diverse range of biological characteristics within these cells. (PMID: 30958800,PMID: 32612951)

实验方案

Product Specific Protocols
WB protocol for ALDH1A3 antibody 29373-1-APDownload protocol
IHC protocol for ALDH1A3 antibody 29373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Exp Clin Cancer Res

Tumor associated macrophages-derived exosomes facilitate hepatocellular carcinoma malignance by transferring lncMMPA to tumor cells and activating glycolysis pathway

Authors - Minghao Xu
View Article