ATG16L1 Monoclonal antibody

ATG16L1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

IF, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G5G7

Cat No : 67943-1-Ig

Print datasheet

Synonyms

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30



经过测试的应用

Positive WB detected inHSC-T6 cells, HepG2 cells, rat lung tissue, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67943-1-Ig targets ATG16L1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF, WB, ELISA
Cited Applications IF, WB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, pig
Immunogen ATG16L1 fusion protein Ag14881 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATG16 autophagy related 16-like 1 (S. cerevisiae)
Synonyms APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30
Calculated Molecular Weight 607 aa, 68 kDa
Observed Molecular Weight68-75 kDa
GenBank Accession NumberBC000061
Gene Symbol ATG16L1
Gene ID (NCBI) 55054
RRIDAB_2918695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ676U5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

实验方案

Product Specific Protocols
WB protocol for ATG16L1 antibody 67943-1-IgDownload protocol
IF protocol for ATG16L1 antibody 67943-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Front Immunol

Porcine cGAS-STING signaling induced autophagy inhibits STING downstream IFN and apoptosis

Authors - Nengwen Xia
humanWB

Epileptic Disord

lncRNA UCA1 induces autophagic gene expression via epigenetic regulation mediated by ATG16L1 and miR-132-3p in SH-SY5Y cells treated with retinoic acid

Authors - Fang Wen
humanWB

Mol Carcinog

Forkhead Box Q1 is a novel regulator of autophagy in breast cancer cells

Authors - Su-Hyeong Kim
humanWB,IF

Dev Cell

V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation

Authors - Yiwei Sun