ATG16L1 Monoclonal antibody

ATG16L1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1G5G7

Cat no : 67943-1-Ig

Print datasheet

Synonyms

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30



经过测试的应用

Positive WB detected inHSC-T6 cells, HepG2 cells, rat lung tissue, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67943-1-Ig targets ATG16L1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, pig
Immunogen ATG16L1 fusion protein Ag14881 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATG16 autophagy related 16-like 1 (S. cerevisiae)
Synonyms APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30
Calculated Molecular Weight 607 aa, 68 kDa
Observed Molecular Weight68-75 kDa
GenBank Accession NumberBC000061
Gene Symbol ATG16L1
Gene ID (NCBI) 55054
RRIDAB_2918695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ676U5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

实验方案

Product Specific Protocols
WB protocol for ATG16L1 antibody 67943-1-IgDownload protocol
IF protocol for ATG16L1 antibody 67943-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Front Immunol

Porcine cGAS-STING signaling induced autophagy inhibits STING downstream IFN and apoptosis

Authors - Nengwen Xia
humanWB

Epileptic Disord

lncRNA UCA1 induces autophagic gene expression via epigenetic regulation mediated by ATG16L1 and miR-132-3p in SH-SY5Y cells treated with retinoic acid

Authors - Fang Wen
humanWB

Mol Carcinog

Forkhead Box Q1 is a novel regulator of autophagy in breast cancer cells

Authors - Su-Hyeong Kim
humanWB,IF

Dev Cell

V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation

Authors - Yiwei Sun
humanWB

Cell Signal

The hsa_circ_0002371/hsa-miR-502-5p/ATG16L1 axis modulates the survival of intracellular Mycobacterium tuberculosis and autophagy in macrophages

Authors - Jinyi Zhang
humanWB,IP

Theranostics

FUNDC1 alleviates doxorubicin-induced cardiotoxicity by restoring mitochondrial-endoplasmic reticulum contacts and blocked autophagic flux

Authors - Weibin He