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AXIN2 Polyclonal antibody

AXIN2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 20540-1-AP

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Synonyms

Axin-2, Axis inhibition protein 2, Axin-like protein, Axin like protein, axin 2



经过测试的应用

Positive WB detected inA549 cells, MDA-MB-453s cells, BxPC-3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy AXIN2 Ready-To-Use IHC Kit. AXIN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20540-1-AP targets AXIN2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen AXIN2 fusion protein Ag14574 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name axin 2
Synonyms Axin-2, Axis inhibition protein 2, Axin-like protein, Axin like protein, axin 2
Calculated Molecular Weight 843 aa, 94 kDa
Observed Molecular Weight 94-100 kDa
GenBank Accession NumberBC006295
Gene Symbol AXIN2
Gene ID (NCBI) 8313
RRIDAB_10694569
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2T1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutation is involved in colorectal cancer. Catalgo#20540-1-AP is a rabbit polyclonal antibdy raised against a 34 amino acid N-terminal of human AXIN2. Observed MW of AXIN2 is from 93-100 kDa (PMID: 11809809; 24607787).

实验方案

Product Specific Protocols
WB protocol for AXIN2 antibody 20540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
humanWB

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Adv Healthc Mater

Ultrathin 2D Titanium Carbide MXene (Ti3 C2 Tx ) Nanoflakes Activate WNT/HIF-1α-Mediated Metabolism Reprogramming for Periodontal Regeneration.

Authors - Di Cui
humanWB

Autophagy

TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.

Authors - Cefan Zhou
humanWB

Theranostics

Targeted inhibition of KDM6 histone demethylases eradicates tumor-initiating cells via enhancer reprogramming in colorectal cancer.

Authors - Junbao Zhang
humanWB

Oncogene

Identification of a novel Calpain-2-SRC feed-back loop as necessity for β-Catenin accumulation and signaling activation in hepatocellular carcinoma.

Authors - Xiao-Lu Ma