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Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (6)

Applications

FC, IF/ICC,IF-P, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 51067-2-AP

Print datasheet

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin



经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells, human placenta tissue, mouse lung tissue, HeLa cells, SH-SY5Y cells, HEK-293 cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, T-47D cells, mouse testis tissue, HeLa cells, HepG2 cells
Positive FC detected inMCF-7 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51067-2-AP targets Beta Catenin in WB, IP, IF, FC, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC, IF/ICC,IF-P, IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC, CoIP, ChIP
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, goat, chicken, sheep, zebrafish, hamster, pig, canine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC058926
Gene Symbol CTNNB1
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2086128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

实验方案

Product Specific Protocols
WB protocol for Beta Catenin antibody 51067-2-APDownload protocol
IHC protocol for Beta Catenin antibody 51067-2-APDownload protocol
IF protocol for Beta Catenin antibody 51067-2-APDownload protocol
IP protocol for Beta Catenin antibody 51067-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,IP,IHC

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
humanIF

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Hepatology

Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.

Authors - Meng Lu
ratWB

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
mouseWB

Hepatology

Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1.

Authors - Sheng-Xian Yuan
humanWB

Nat Commun

CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.

Authors - Yuan Fang