Featured Product
KD/KO Validated

GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Publications

356

Cat no : 22104-1-AP

Print datasheet

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta, GSK-3beta, GSK3β

验证数据展示

WB analysis of A549 using 22104-1-AP
WB result of GSK3B antibody (22104-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.
WB analysis using 22104-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 22104-1-AP
IP result of anti-GSK3B (IP:22104-1-AP, 4ug; Detection:22104-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human lung cancer using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with GSK3B antibody included
IHC staining of human lung cancer using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with GSK3B antibody included
IF Staining of MCF-7 using 22104-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (22104-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 22104-1-AP

Size

点击咨询在线客服

经过测试的应用

Positive WB detected in	HeLa cells, A549 cells, mouse brain tissue, mouse kidney tissue, mouse ovary tissue, LNCaP cells, mouse testis tissue, rat brain tissue, PC-3 cells, mouse thymus tissue, rat thymus tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lung cancer tissue, human prostate hyperplasia tissue, human testis tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 10 publications below
WB	See 345 publications below
IHC	See 15 publications below
IF	See 11 publications below
IP	See 7 publications below
CoIP	See 6 publications below
RIP	See 1 publications below

产品信息

22104-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, RIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish, bovine, hamster
Immunogen	GSK3B fusion protein Ag17320 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	glycogen synthase kinase 3 beta
Synonyms	GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta, GSK-3beta, GSK3β
Calculated Molecular Weight	433 aa, 48 kDa
Observed Molecular Weight	46-48 kDa
GenBank Accession Number	BC000251
Gene Symbol	GSK3B
Gene ID (NCBI)	2932
RRID	AB_2878997
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49841
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution. This antibody recognize the C-terminal of GSK3B.

实验方案

Product Specific Protocols
WB protocol for GSK3B antibody 22104-1-AP	Download protocol
IHC protocol for GSK3B antibody 22104-1-AP	Download protocol
IF protocol for GSK3B antibody 22104-1-AP	Download protocol
IP protocol for GSK3B antibody 22104-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nature CFIm25 links alternative polyadenylation to glioblastoma tumour suppression. Authors - Chioniso P Masamha View Article
human	WB	Mol Cancer Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1. Authors - Shan Xing View Article
human	WB	Cell Rep Med Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition Authors - Yu Chang View Article
human	WB	Nat Commun Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. Authors - Wenjun Wang View Article
zebrafish	WB	Nat Commun ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling. Authors - Xiaoyun Sun View Article
rat	WB	Hepatology Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients. Authors - Daimin Xiang View Article

产品中心

应用中心

研究领域

cGMP 级蛋白

技术支持

促销活动

关于我们

抗体

免疫分析

PTG-Lab免疫学实验试剂

PTG-Cell细胞实验试剂

细胞因子&活性蛋白

重组蛋白

纳米抗体相关产品

空间多组学

表观遗传领域相关产品

研究领域

GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis of A549 using 22104-1-AP

WB analysis using 22104-1-AP

IP experiment of mouse brain using 22104-1-AP

IHC staining of human lung cancer using 22104-1-AP

IHC staining of human lung cancer using 22104-1-AP

IF Staining of MCF-7 using 22104-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1.

Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.

Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.