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KD/KO Validated

E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Publications

1619

Cat No : 20874-1-AP

Print datasheet

Synonyms

Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin

验证数据展示

WB analysis of A431 using 20874-1-AP
WB result of E-cadherin antibody (20874-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.
IF Staining of mouse embryo using 20874-1-AP
E14.5 FFPE mouse embryo section stained for E-cadherin (red, Cat. No 20874-1-AP) and alpha tubulin (green, Cat. CL488-66031). Image credit: @Immunofluorescence on Instagram
WB analysis using 20874-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.
IF Staining of mouse small intestine using 20874-1-AP
FFPE adult mouse small intestine stained for E-cadherin (white, 20874-1-AP5), LYVE1 (green), and alpha-smooth muscle actin (red). E-cadherin marks intestinal epithelial cells, which regulate nutrient absorption. LYVE1 stains gut lymphatics, which absorb fat. Alpha-smooth muscle actin stains perivascular smooth muscle cells and the surrounding smooth muscle, which help propel food in the gut. Image credit: @Immunofluorescence on Instagram.
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FFPE adult mouse small intestine stained for E-cadherin (white, 20874-1-AP5), LYVE1 (green), and alpha-smooth muscle actin (red). E-cadherin marks intestinal epithelial cells, which regulate nutrient absorption. LYVE1 stains gut lymphatics, which absorb fat. Alpha-smooth muscle actin stains perivascular smooth muscle cells and the surrounding smooth muscle, which help propel food in the gut. Image credit: @Immunofluorescence on Instagram.
IHC staining of mouse colon using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 20874-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 20874-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 20874-1-AP
1X10^6 HepG2 cells were stained with .2ug E-cadherin antibody (20874-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP experiment of A431 using 20874-1-AP
IP Result of anti-E-cadherin (IP:20874-1-AP, 3ug; Detection:20874-1-AP 1:1000) with A431 cells lysate 3000ug.

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Cat No. 20874-1-AP

Size

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经过测试的应用

Positive WB detected in	A431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells
Positive IP detected in	A431 cells
Positive IHC detected in	mouse colon tissue, human breast cancer tissue, human colon tissue, mouse skin tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	MCF-7 cells, A431 cells, mouse small intestine tissue, mouse embryo tissue, human breast cancer tissue
Positive FC detected in	HepG2 cells

Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:5000-1:20000
Immunofluorescence (IF)	IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

发表文章中的应用

KD/KO	See 3 publications below
WB	See 1424 publications below
IHC	See 264 publications below
IF	See 305 publications below
IP	See 2 publications below
FC	See 3 publications below
CoIP	See 6 publications below

产品信息

20874-1-AP targets E-cadherin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	FC, IF, IHC, IP, WB, ELISA
Cited Applications	CoIP, FC, IF, IHC, IP, WB
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish, pig, canine, bovine
Immunogen	E-cadherin fusion protein Ag14973 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	cadherin 1, type 1, E-cadherin (epithelial)
Synonyms	Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin
Calculated molecular weight	882 aa, 97 kDa
Observed molecular weight	120-125 kDa
GenBank accession number	BC141838
Gene symbol	CDH1
Uniprot	P12830
Gene ID (NCBI)	999
RRID	AB_10697811
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

实验方案

Product Specific Protocols
WB protocol for E-cadherin antibody 20874-1-AP	Download protocol
IHC protocol for E-cadherin antibody 20874-1-AP	Download protocol
IF protocol for E-cadherin antibody 20874-1-AP	Download protocol
IP protocol for E-cadherin antibody 20874-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Science Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice. Authors - Yanpu Chen View Article
human,mouse	WB,IHC	Mol Cancer lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2 Authors - Jin Wang View Article
human, rat, mouse	WB	Sci Transl Med MG53-mediated cell membrane repair protects against acute kidney injury. Authors - Pu Duann View Article
mouse	WB	ACS Nano Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment. Authors - Han Lin View Article
human,mouse	WB,IHC,IF,FC	Nat Commun TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrity Authors - Sujin Kang View Article
human	WB	Nat Commun UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis Authors - Jeonghyun Kim View Article

E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

验证数据展示

WB analysis of A431 using 20874-1-AP

IF Staining of mouse embryo using 20874-1-AP

WB analysis using 20874-1-AP

IF Staining of mouse small intestine using 20874-1-AP

IHC staining of mouse colon using 20874-1-AP

IHC staining of mouse colon using 20874-1-AP

IF Staining of MCF-7 using 20874-1-AP

FC experiment of HepG2 using 20874-1-AP

IP experiment of A431 using 20874-1-AP

经过测试的应用

Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice.

lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2

MG53-mediated cell membrane repair protects against acute kidney injury.

Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.

TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrity

UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis

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