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E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20874-1-AP

Print datasheet

Synonyms

Arc 1, Cadherin 1, CD324, CDH1, CDHE, E cadherin, ECAD, E-cad, E-cadherin, LCAM, UVO, Uvomorulin



经过测试的应用

Positive WB detected inA431 cells, mouse testis tissue, DU 145 cells, MCF-7 cells, T-47D cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human hepatocirrhosis tissue, mouse liver tissue, human skin tissue, mouse colon tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, mouse small intestine tissue, human breast cancer tissue, mouse embryo tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 20874-1-AP is E-cadherin Fusion Protein expressed in E. coli.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications CoIP, FC, IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, canine, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag14973
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight 882 aa, 97 kDa
Observed molecular weight 120-125 kDa
GenBank accession numberBC141838
Gene symbol CDH1
UniprotP12830
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

实验方案

Product Specific Protocols
WB protocol for E-cadherin antibody 20874-1-APDownload protocol
IHC protocol for E-cadherin antibody 20874-1-APDownload protocol
IF protocol for E-cadherin antibody 20874-1-APDownload protocol
IP protocol for E-cadherin antibody 20874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

P53-induced miR-1249 inhibits tumor growth, metastasis, and angiogenesis by targeting VEGFA and HMGA2.

Authors - Xiaoxiang Chen
humanWB

Cell Death Dis

miR-340-FHL2 axis inhibits cell growth and metastasis in ovarian cancer.

Authors - Zheng Huang
humanWB,IHC

J Cell Biochem

PKM2-regulated STAT3 promotes esophageal squamous cell carcinoma progression via TGF-β1-induced EMT.

Authors - Rong Ma
humanWB,IF

Front Physiol

Keratinocytes Isolated From Individual Cleft Lip/Palate Patients Display Variations in Their Differentiation Potential in vitro.

Authors - Martin Degen
humanWB

J Cancer

Lyn Kinase Promotes the Proliferation of Malignant Melanoma Cells through Inhibition of Apoptosis and Autophagy via the PI3K/Akt Signaling Pathway.

Authors - Qianqian Zhang
humanWB

Carcinogenesis

Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.

Authors - Haoran Li