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- KD/KO Validated
E-cadherin Polyclonal antibody
E-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA and More (3)
Conjugate
Unconjugated
1997
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells |
Positive IP detected in | A431 cells |
Positive IHC detected in | mouse colon tissue, human breast cancer tissue, human colon tissue, human prostate cancer tissue, mouse skin tissue, rat colon tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse colon tissue, human breast cancer tissue, mouse small intestine tissue |
Positive IF-Fro detected in | mouse colon tissue |
Positive IF/ICC detected in | MCF-7 cells, A431 cells |
Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:200000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20874-1-AP targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, Cell treatment |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, canine, zebrafish, bovine |
Immunogen | E-cadherin fusion protein Ag14973 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
Synonyms | CDH1, CD324, CAM 120/80, Cadherin-1, Cadherin 1 |
Calculated Molecular Weight | 882 aa, 97 kDa |
Observed Molecular Weight | 120-125 kDa |
GenBank Accession Number | BC141838 |
Gene Symbol | E-cadherin |
Gene ID (NCBI) | 999 |
RRID | AB_10697811 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P12830 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for E-cadherin antibody 20874-1-AP | Download protocol |
IHC protocol for E-cadherin antibody 20874-1-AP | Download protocol |
IF protocol for E-cadherin antibody 20874-1-AP | Download protocol |
IP protocol for E-cadherin antibody 20874-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice. | ||
Mol Cancer lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2 | ||
Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration | ||
ACS Nano Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment. | ||
Nat Commun TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrity |