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验证数据展示

  • IF Staining of U2OS using CL488-83082-4

    IF Staining of U2OS using CL488-83082-4

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 CA11 antibody (CL488-83082-4, Clone: 230390F7 ) at dilution of 1:200.

  • FC experiment of A431 using CL488-83082-4

    FC experiment of A431 using CL488-83082-4

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CA11 Recombinant antibody (CL488-83082-4, Clone:230390F7)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated CA11 Recombinant antibody

CA11 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83082-4

产品说明书

CloneNo. 230390F7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Carbonic anhydrase-related protein 11, carbonic anhydrase XI, CA XI, CA RP XI, CA RP II

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 83082-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83082-4 targets CA11 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 CA11 fusion protein Ag33373 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carbonic anhydrase XI
别名 Carbonic anhydrase-related protein 11, carbonic anhydrase XI, CA XI, CA RP XI, CA RP II
计算分子量 36 kDa
GenBank蛋白编号BC002662
基因名称 CA11
Gene ID (NCBI) 770
RRIDAB_3673181
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75493
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA11(Carbonic anhydrase-related protein 11) is also named CARP2, and CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical, and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CA11 antibody CL488-83082-4Download protocol
FC protocol for CL Plus 488 CA11 antibody CL488-83082-4Download protocol
Standard Protocols
Click here to view our Standard Protocols