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c-Cbl Polyclonal antibody

c-Cbl Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 25818-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA and More (2)

CBL, EC:2.3.2.27, E3 ubiquitin-protein ligase CBL, CBL2, Casitas B-lineage lymphoma proto-oncogene

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, Raji cells, Ramos cells, Romas cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CBL Ready-To-Use IHC Kit. CBL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25818-1-AP targets c-Cbl in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 c-Cbl fusion protein Ag23033 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cas-Br-M (murine) ecotropic retroviral transforming sequence
别名 CBL, EC:2.3.2.27, E3 ubiquitin-protein ligase CBL, CBL2, Casitas B-lineage lymphoma proto-oncogene
计算分子量 906 aa, 100 kDa
观测分子量 100-120 kDa
GenBank蛋白编号BC132733
基因名称 CBL
Gene ID (NCBI) 867
RRIDAB_2880252
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22681
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

实验方案

Product Specific Protocols
WB protocol for c-Cbl antibody 25818-1-APDownload protocol
IHC protocol for c-Cbl antibody 25818-1-APDownload protocol
IP protocol for c-Cbl antibody 25818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Hepatology

PMID: 29729186

Authors - Huixia Zhang
  • KD Validated
humanWB,IF,CoIP

Cell Rep

Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis

Authors - Huan Li
  • KD Validated
humanWB

Cell Death Dis

ZC3H15 promotes glioblastoma progression through regulating EGFR stability.

Authors - Jianbing Hou
humanWB

Cell Commun Signal

Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells

Authors - Limei Xu
mouseWB

Front Med

Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ

Authors - Fei Xu
humanWB

Oncogene

AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression

Authors - Sicheng Wan
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