验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, Raji cells, Ramos cells, Romas cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25818-1-AP targets c-Cbl in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | c-Cbl fusion protein Ag23033 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
别名 | CBL, EC:2.3.2.27, E3 ubiquitin-protein ligase CBL, CBL2, Casitas B-lineage lymphoma proto-oncogene |
计算分子量 | 906 aa, 100 kDa |
观测分子量 | 100-120 kDa |
GenBank蛋白编号 | BC132733 |
基因名称 | CBL |
Gene ID (NCBI) | 867 |
RRID | AB_2880252 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P22681 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
实验方案
Product Specific Protocols | |
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WB protocol for c-Cbl antibody 25818-1-AP | Download protocol |
IHC protocol for c-Cbl antibody 25818-1-AP | Download protocol |
IP protocol for c-Cbl antibody 25818-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis
| ||
Cell Death Dis ZC3H15 promotes glioblastoma progression through regulating EGFR stability. | ||
Cell Commun Signal Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells | ||
Front Med Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ | ||