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CoraLite® Plus 488 Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

N418

Cat no : CL488-65130

Print datasheet

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

验证数据展示

  • FC experiment of mouse splenocytes using CL488-65130

    FC experiment of mouse splenocytes using CL488-65130

    1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11c (CL488-65130, Clone: N418) or 0.25 ug isotype control. Cells were not fixed.

  • IF Staining of mouse splenocytes using CL488-65130

    IF Staining of mouse splenocytes using CL488-65130

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD11c antibody (CL488-65130, Clone: N418 ) at dilution of 1:400.

经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65130-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65130 targets CD11c in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity Mouse
Immunogen Mouse spleen dendritic cells 种属同源性预测
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Full Name integrin alpha X
Synonyms AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418
GenBank Accession NumberBC167225
Gene Symbol CD11c
Gene ID (NCBI) 16411
RRIDAB_2883193
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ9QXH4
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD11c antibody CL488-65130Download protocol
FC protocol for CL Plus 488 CD11c antibody CL488-65130Download protocol
Standard Protocols
Click here to view our Standard Protocols