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验证数据展示

  • FC experiment of mouse splenocytes using CL594-65130

    FC experiment of mouse splenocytes using CL594-65130

    1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse MHC Class II (I-A/I-E) and 0.2 ug CoraLite®594 Anti-Mouse CD11c (CL594-65130, Clone:N418) or 0.2 ug Isotype Control. Cells were not fixed.

CoraLite®594 Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for FC
Cat No. CL594-65130

Print datasheet

CloneNo. N418

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65130-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65130 targets CD11c in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse spleen dendritic cells 种属同源性预测
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Full Name integrin alpha X
Synonyms AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418
GenBank Accession NumberBC167225
Gene Symbol CD11c
Gene ID (NCBI) 16411
RRIDAB_3084669
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9QXH4
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

实验方案

Product Specific Protocols
FC protocol for CL594 CD11c antibody CL594-65130Download protocol
Standard Protocols
Click here to view our Standard Protocols