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CoraLite® Plus 488 Anti-Mouse CD134/OX40 (OX-86)

CD134/OX40 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

OX-86

Cat no : CL488-65136

Print datasheet

Synonyms

Tnfrsf4, CD134, Txgp1, OX-86, Ox40

验证数据展示

  • FC experiment of mouse splenocytes using CL488-65136

    FC experiment of mouse splenocytes using CL488-65136

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD134 (OX-86) (CL488-65136, Clone:OX-86) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

经过测试的应用

Positive FC detected inAnti-CD3/CD28 stimulated mouse splenocytes, Con-A stimulated mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65136-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65136 targets CD134/OX40 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 4
Synonyms Tnfrsf4, CD134, Txgp1, OX-86, Ox40
GenBank Accession NumberBC065782
Gene Symbol CD134
Gene ID (NCBI) 22163
RRIDAB_2919267
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD134/OX40 antibody CL488-65136Download protocol
Standard Protocols
Click here to view our Standard Protocols