验证数据展示
经过测试的应用
Positive FC detected in | Anti-CD3/CD28 stimulated mouse splenocytes, Con-A stimulated mouse splenocytes |
推荐稀释比
应用 | 推荐稀释比 |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-65136 targets CD134/OX40 in FC applications and shows reactivity with mouse samples.
经测试应用 | FC Application Description |
经测试反应性 | mouse |
免疫原 | N/A 种属同源性预测 |
宿主/亚型 | Rat / IgG1, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | tumor necrosis factor receptor superfamily, member 4 |
别名 | Tnfrsf4, CD134, Txgp1, OX-86, Ox40 |
GenBank蛋白编号 | BC065782 |
基因名称 | CD134 |
Gene ID (NCBI) | 22163 |
RRID | AB_2919267 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Affinity purification |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD134/OX40 antibody CL488-65136 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |