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CD19 Monoclonal antibody, PBS Only

CD19 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C10A1

Cat no : 66298-1-PBS

Print datasheet

Synonyms

CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4, B lymphocyte antigen CD19

验证数据展示

  • WB analysis of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    WB analysis of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • WB analysis of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    WB analysis of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    IF Staining of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (66298-1-Ig, Clone: 1C10A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

产品信息

66298-1-PBS targets CD19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CD19 fusion protein Ag19945 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD19 molecule
Synonyms CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4, B lymphocyte antigen CD19
Calculated Molecular Weight 556 aa, 61 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
RRIDAB_2881681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15391
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).