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Anti-Human CD235a (HIR2)

CD235a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

HIR2

Cat no : 65174-1-Ig

Print datasheet

Synonyms

Glycophorin A, GPSAT, GpMiIII, GPErik, GPA

验证数据展示

  • FC experiment of human peripheral blood using 65174-1-Ig

    FC experiment of human peripheral blood using 65174-1-Ig

    100 ul human peripheral blood (diluted 1:15 with PBS) was surface stained with 0.2 ug Anti-Human CD235a (65174-1-Ig, Clone:HIR2) or Mouse IgG2b Isotype Control (65128-1-Ig, Clone: MPC-11) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed.

  • FC experiment of human peripheral blood using 65174-1-Ig

    FC experiment of human peripheral blood using 65174-1-Ig

    100 ul human peripheral blood (diluted 1:15 with PBS) was surface stained with 0.2 ug Anti-Human CD235a (65174-1-Ig, Clone:HIR2) or Mouse IgG2b Isotype Control (65128-1-Ig, Clone: MPC-11) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65174-1-Ig targets CD235a in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Synthetic peptide (Human, N-terminal) 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name glycophorin A (MNS blood group)
Synonyms Glycophorin A, GPSAT, GpMiIII, GPErik, GPA
Calculated Molecular Weight 150 aa, 16 kDa
GenBank Accession NumberBC005319
Gene Symbol Glycophorin A
Gene ID (NCBI) 2993
RRIDAB_2918449
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP02724
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
FC protocol for CD235a antibody 65174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols