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CoraLite® Plus 594 Anti-Human CD235a (HIR2)

CD235a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

HIR2

Cat no : CL594-65174

Print datasheet

Synonyms

Glycophorin A, GPSAT, GpMiIII, GPErik, GPA

验证数据展示

  • FC experiment of human blood using CL594-65174

    FC experiment of human blood using CL594-65174

    100ul human whole blood (diluted 1:15 with PBS) was surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD235a (CL594-65174, Clone:HIR2) or CoraLite®594 Mouse IgG2b Isotype Control (CL594-65128, Clone: MPC-11). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman blood
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65174-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65174 targets CD235a in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Synthetic peptide (Human, N-terminal) 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name glycophorin A (MNS blood group)
Synonyms Glycophorin A, GPSAT, GpMiIII, GPErik, GPA
Calculated Molecular Weight 150 aa, 16 kDa
GenBank Accession NumberBC005319
Gene Symbol Glycophorin A
Gene ID (NCBI) 2993
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodUnknow
UNIPROT IDP02724
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
FC protocol for CL Plus 594 CD235a antibody CL594-65174Download protocol
Standard Protocols
Click here to view our Standard Protocols