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验证数据展示

  • FC experiment of human whole blood using CL488-65174

    FC experiment of human whole blood using CL488-65174

    10 ul human whole blood was surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD235a (HIR2) (CL488-65174) or CoraLite® Plus 488 Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

  • FC experiment of human whole blood using CL488-65174

    FC experiment of human whole blood using CL488-65174

    10 ul human whole blood was surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD235a (HIR2) (CL488-65174) or CoraLite® Plus 488 Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

CoraLite® Plus 488 Anti-Human CD235a (HIR2)

CD235a Monoclonal Antibody for FC
Cat No. CL488-65174

产品说明书

CloneNo. HIR2

宿主/亚型

Mouse / IgG2b, kappa

种属反应性

Human

应用

FC

别名

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman whole blood
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65174-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65174 targets CD235a in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 Synthetic peptide (Human, N-terminal) 种属同源性预测
宿主/亚型 Mouse / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 glycophorin A (MNS blood group)
别名 CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha
计算分子量 150 aa, 16 kDa
GenBank蛋白编号BC005319
基因名称 Glycophorin A
Gene ID (NCBI) 2993
RRIDAB_3672869
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP02724
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD235a antibody CL488-65174Download protocol
Standard Protocols
Click here to view our Standard Protocols