验证数据展示
经过测试的应用
| Positive FC detected in | human red blood cells |
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
FITC-65174 targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | human |
| Immunogen | Synthetic peptide (Human, N-terminal) 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glycophorin A (MNS blood group) |
| Synonyms | Glycophorin A, CD235a, GpMiIII, GPErik, GPA |
| Calculated Molecular Weight | 150 aa, 16 kDa |
| GenBank Accession Number | BC005319 |
| Gene Symbol | Glycophorin A |
| Gene ID (NCBI) | 2993 |
| RRID | AB_2883814 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | P02724 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus Glycophorin A/CD235a antibody FITC-65174 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |