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Cardinal Red™ Anti-Human CD235a (HIR2)

CD235a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

HIR2

Cat no : CR-65174

Print datasheet

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

验证数据展示

  • FC experiment of human whole blood using CR-65174

    FC experiment of human whole blood using CR-65174

    10 ul human whole blood was surface stained with 5 ul Cardinal Red™ Anti-Human CD235a (HIR2) (CR-65174) or Cardinal Red™ Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

  • FC experiment of human whole blood using CR-65174

    FC experiment of human whole blood using CR-65174

    10 ul human whole blood was surface stained with 5 ul Cardinal Red™ Anti-Human CD235a (HIR2) (CR-65174) or Cardinal Red™ Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

经过测试的应用

Positive FC detected inhuman whole blood
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-65174 targets CD235a in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Synthetic peptide (Human, N-terminal) 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name glycophorin A (MNS blood group)
Synonyms CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha
Calculated Molecular Weight 150 aa, 16 kDa
GenBank Accession NumberBC005319
Gene Symbol Glycophorin A
Gene ID (NCBI) 2993
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP02724
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
FC protocol for Cardinal Red™ CD235a antibody CR-65174Download protocol
Standard Protocols
Click here to view our Standard Protocols