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PE Anti-Mouse PD-1/CD279 (J43.1)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

J43.1

Cat no : PE-65120

Print datasheet

Synonyms

CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1

验证数据展示

  • FC experiment of mouse splenocytes using PE-65120

    FC experiment of mouse splenocytes using PE-65120

    1X10^6 unstimulated and anti-CD3/CD28-stimulated (3 days) mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 and 0.5 ug PE Anti-Mouse PD-1/CD279 (PE-65120, Clone: J43.1). Cells were not fixed.

经过测试的应用

Positive FC detected inanti-CD3/CD28-stimulated mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65120 targets PD-1/CD279 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen B12 cells, mouse PD-1 cDNA-transfected BHK cells 种属同源性预测
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1
GenBank Accession NumberBC119179
Gene Symbol PD-1
Gene ID (NCBI) 18566
RRIDAB_2883908
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ02242
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for PE PD-1/CD279 antibody PE-65120Download protocol
Standard Protocols
Click here to view our Standard Protocols