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验证数据展示

  • WB analysis using 16355-1-AP

    WB analysis using 16355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16355-1-AP (CYP2C9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16355-1-AP

    IHC staining of human liver using 16355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16355-1-AP (CYP2C9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16355-1-AP

    IHC staining of human liver using 16355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16355-1-AP (CYP2C9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CYP2C9 Polyclonal antibody

CYP2C9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16355-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

(R) limonene 6 monooxygenase, (S) limonene 6 monooxygenase, (S) limonene 7 monooxygenase, CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, Cytochrome P 450MP, Cytochrome P450 2C9, Cytochrome P450 MP 4, Cytochrome P450 MP 8, Cytochrome P450 PB 1, P450IIC9, S mephenytoin 4 hydroxylase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, SMMC-7721 cells, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16355-1-AP targets CYP2C9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP2C9 fusion protein Ag9566 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 2, subfamily C, polypeptide 9
Synonyms (R) limonene 6 monooxygenase, (S) limonene 6 monooxygenase, (S) limonene 7 monooxygenase, CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, Cytochrome P 450MP, Cytochrome P450 2C9, Cytochrome P450 MP 4, Cytochrome P450 MP 8, Cytochrome P450 PB 1, P450IIC9, S mephenytoin 4 hydroxylase
Calculated Molecular Weight162aa,18 kDa; 490aa,56 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC020754
Gene Symbol CYP2C9
Gene ID (NCBI) 1559
RRIDAB_3085487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11712
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP2C9 (Cytochrome P450 Family 2 Subfamily C Member 9) is a crucial cytochrome P450 enzyme, which plays a significant role in the metabolism, by oxidation, of both xenobiotic and endogenous compounds. CYP2C9 is primarily expressed in the liver, and the expression level is reported to be the second highest among CYP isoforms(PMID: 20150829).

实验方案

Product Specific Protocols
WB protocol for CYP2C9 antibody 16355-1-APDownload protocol
IHC protocol for CYP2C9 antibody 16355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols