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验证数据展示

  • WB analysis using 16355-1-AP

    WB analysis using 16355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16355-1-AP (CYP2C9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16355-1-AP

    IHC staining of human liver using 16355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16355-1-AP (CYP2C9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16355-1-AP

    IHC staining of human liver using 16355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16355-1-AP (CYP2C9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CYP2C9 Polyclonal antibody

CYP2C9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16355-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

(R) limonene 6 monooxygenase, (S) limonene 6 monooxygenase, (S) limonene 7 monooxygenase, CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, Cytochrome P 450MP, Cytochrome P450 2C9, Cytochrome P450 MP 4, Cytochrome P450 MP 8, Cytochrome P450 PB 1, P450IIC9, S mephenytoin 4 hydroxylase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, SMMC-7721 cells, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16355-1-AP targets CYP2C9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CYP2C9 fusion protein Ag9566 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily C, polypeptide 9
别名 (R) limonene 6 monooxygenase, (S) limonene 6 monooxygenase, (S) limonene 7 monooxygenase, CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, Cytochrome P 450MP, Cytochrome P450 2C9, Cytochrome P450 MP 4, Cytochrome P450 MP 8, Cytochrome P450 PB 1, P450IIC9, S mephenytoin 4 hydroxylase
计算分子量162aa,18 kDa; 490aa,56 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC020754
基因名称 CYP2C9
Gene ID (NCBI) 1559
RRIDAB_3085487
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11712
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP2C9 (Cytochrome P450 Family 2 Subfamily C Member 9) is a crucial cytochrome P450 enzyme, which plays a significant role in the metabolism, by oxidation, of both xenobiotic and endogenous compounds. CYP2C9 is primarily expressed in the liver, and the expression level is reported to be the second highest among CYP isoforms(PMID: 20150829).

实验方案

Product Specific Protocols
WB protocol for CYP2C9 antibody 16355-1-APDownload protocol
IHC protocol for CYP2C9 antibody 16355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols