验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10365-1-AP targets DKK3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, canine |
| 免疫原 | DKK3 fusion protein Ag0417 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dickkopf homolog 3 (Xenopus laevis) |
| 别名 | Dkk-3, Dkk 3, Dickkopf-related protein 3, Dickkopf-3, Dickkopf3 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 38-55 kDa |
| GenBank蛋白编号 | BC007660 |
| 基因名称 | DKK3 |
| Gene ID (NCBI) | 27122 |
| RRID | AB_2261615 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBP4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DKK3 antibody 10365-1-AP | Download protocol |
| IHC protocol for DKK3 antibody 10365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Sci Transl Med Suppression of stromal-derived Dickkopf-3 (DKK3) inhibits tumor progression and prolongs survival in pancreatic ductal adenocarcinoma.
| ||
J Clin Invest Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging. | ||
J Cell Mol Med Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats. | ||
Mol Carcinog Wnt antagonist DICKKOPF-3 (Dkk-3) induces apoptosis in human renal cell carcinoma. |
