ChromoTek Dnmt1-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagGFP2.




IF, Live cell imaging

Vector type

Mammalian expression vector



Cat No : dcg


Chromobody, Dmnt1 Chromobody

Product Information

DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagGFP2.

DescriptionThe Dnmt1-Chromobody visualizes endogenous Dnmt1 in real-time during cell cycle progression.

• Trace dynamic changes during the cell cycle in real time

• Monitor the distribution of an endogenous cell cycle marker protein without overexpression artifacts or cytotoxic effects from overexpressed fluorescent protein fusion Dnmt1

ApplicationsLive cell imaging
SpecificityDnmt1 (DNA (cytosine-5)-methyltransferase 1), tested in human cells
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SequenceWith the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis
Storage ConditionShipped at ambient temperature. Store at -20 °C



Nat Commun

Nanobody-mediated control of gene expression and epigenetic memory.

Authors - Mike V Van

Biotechnol J

Tracing endogenous proteins in living cells through electrotransfer of mRNA encoding chromobodies

Authors - Théo Juncker