Drebrin Monoclonal antibody
Drebrin Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A12H1
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, pig brain tissue, HEK-293 cells, PC-12 cells, Neuro-2a cells, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67589-1-Ig targets Drebrin in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | IF-P, IHC, WB, ELISA Application Description |
| Tested Reactivity | Human, mouse, rat, pig |
| Immunogen | Drebrin fusion protein Ag22635 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | drebrin 1 |
| Synonyms | D0S117E, DBN1, DKFZp434D064, Drebrin, drebrin 1 |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC007567 |
| Gene Symbol | DBN1 |
| Gene ID (NCBI) | 1627 |
| ENSEMBL Gene ID | ENSG00000113758 |
| RRID | AB_2882797 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16643 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Drebrin antibody 67589-1-Ig | Download protocol |
| IHC protocol for Drebrin antibody 67589-1-Ig | Download protocol |
| IF protocol for Drebrin antibody 67589-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
