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CoraLite® Plus 647-conjugated Drebrin Monoclonal antibody

Drebrin Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1A12H1

Cat no : CL647-67589

Print datasheet

Synonyms

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 1A12H1

验证数据展示

  • IF Staining of SH-SY5Y using CL647-67589

    IF Staining of SH-SY5Y using CL647-67589

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 647 Drebrin antibody (CL647-67589, Clone: 1A12H1 ) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL647-67589

    FC experiment of SH-SY5Y using CL647-67589

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Drebrin (CL647-67589, Clone:1A12H1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67589-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67589 targets Drebrin in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen Drebrin fusion protein Ag22635 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name drebrin 1
Synonyms DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 1A12H1
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC007567
Gene Symbol Drebrin
Gene ID (NCBI) 1627
ENSEMBL Gene IDENSG00000113758
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16643
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Drebrin antibody CL647-67589Download protocol
Standard Protocols
Click here to view our Standard Protocols