CoraLite®594-conjugated Drebrin Monoclonal antibody
Drebrin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1A12H1
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67589-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67589 targets Drebrin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IF-P, FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Drebrin fusion protein Ag22635 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | drebrin 1 |
| Synonyms | DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 1A12H1 |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC007567 |
| Gene Symbol | Drebrin |
| Gene ID (NCBI) | 1627 |
| ENSEMBL Gene ID | ENSG00000113758 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16643 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Drebrin antibody CL594-67589 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
